gene (human) scn2a Search Results


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( a ) Combined brightfield and fluorescence image of a whole-cell recording from a <t>SCN2A-EGFP</t> + HEK-293 cell (green). Scalebar, 10 μm. ( b ) Example traces of I Na recorded in response to depolarizing voltage command potentials ( bottom ) for a non-transfected ( top ) and SCN2A-EGFP + cell ( middle ), scale bars indicate 0.5 nA and 5 ms. ( c ) Peak I Na traces before (black) and after TTX application (red) in response to a depolarizing voltage step ( bottom ), scale bars indicate 100 pA and 10 ms. ( d ) Example current traces of steady-state inactivation ( left ) and activation protocols ( right ), scale bars indicated 100 pA and 5 ms. ( e ) Population data for steady-state activation and inactivation curves, circles and error bars indicate mean ± s.e.m. Lines represent Boltzmann fits to the mean data. ( f ) Schematic of the experiment: a SCN2A-EGFP + HEK-293 cell was recorded in voltage-clamp and filled with OGB-1 (0.1 mM) after which a train of depolarizing pules (–120 to –30 mV, 200 Hz, 1 s) was applied, top to bottom , ΔF/F before and after bath application of 1 μM TTX (blue), the recorded currents and voltage command potentials, right , magnification of the first three action currents, scale bars from top to bottom represent 1% ΔF/F, 0.5 nA, 100 mV, 500 ms and 10 ms. ( g ) Color-coded average ΔF/F of 100 frames before onset ( left ) and at the end ( right ) of the voltage command, scale bar indicates 10 μm. ( h ) OGB-1 ΔF/F is significantly higher in control ( n = 7) than after application of TTX ( n = 4), one-tailed Mann Whitney test, p=0.0121. Data available in . Figure 7—source data 1. Na V 1.2 channels mediate a Ca 2+ influx.
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( a ) Combined brightfield and fluorescence image of a whole-cell recording from a SCN2A-EGFP + HEK-293 cell (green). Scalebar, 10 μm. ( b ) Example traces of I Na recorded in response to depolarizing voltage command potentials ( bottom ) for a non-transfected ( top ) and SCN2A-EGFP + cell ( middle ), scale bars indicate 0.5 nA and 5 ms. ( c ) Peak I Na traces before (black) and after TTX application (red) in response to a depolarizing voltage step ( bottom ), scale bars indicate 100 pA and 10 ms. ( d ) Example current traces of steady-state inactivation ( left ) and activation protocols ( right ), scale bars indicated 100 pA and 5 ms. ( e ) Population data for steady-state activation and inactivation curves, circles and error bars indicate mean ± s.e.m. Lines represent Boltzmann fits to the mean data. ( f ) Schematic of the experiment: a SCN2A-EGFP + HEK-293 cell was recorded in voltage-clamp and filled with OGB-1 (0.1 mM) after which a train of depolarizing pules (–120 to –30 mV, 200 Hz, 1 s) was applied, top to bottom , ΔF/F before and after bath application of 1 μM TTX (blue), the recorded currents and voltage command potentials, right , magnification of the first three action currents, scale bars from top to bottom represent 1% ΔF/F, 0.5 nA, 100 mV, 500 ms and 10 ms. ( g ) Color-coded average ΔF/F of 100 frames before onset ( left ) and at the end ( right ) of the voltage command, scale bar indicates 10 μm. ( h ) OGB-1 ΔF/F is significantly higher in control ( n = 7) than after application of TTX ( n = 4), one-tailed Mann Whitney test, p=0.0121. Data available in . Figure 7—source data 1. Na V 1.2 channels mediate a Ca 2+ influx.

Journal: eLife

Article Title: Ca 2+ entry through Na V channels generates submillisecond axonal Ca 2+ signaling

doi: 10.7554/eLife.54566

Figure Lengend Snippet: ( a ) Combined brightfield and fluorescence image of a whole-cell recording from a SCN2A-EGFP + HEK-293 cell (green). Scalebar, 10 μm. ( b ) Example traces of I Na recorded in response to depolarizing voltage command potentials ( bottom ) for a non-transfected ( top ) and SCN2A-EGFP + cell ( middle ), scale bars indicate 0.5 nA and 5 ms. ( c ) Peak I Na traces before (black) and after TTX application (red) in response to a depolarizing voltage step ( bottom ), scale bars indicate 100 pA and 10 ms. ( d ) Example current traces of steady-state inactivation ( left ) and activation protocols ( right ), scale bars indicated 100 pA and 5 ms. ( e ) Population data for steady-state activation and inactivation curves, circles and error bars indicate mean ± s.e.m. Lines represent Boltzmann fits to the mean data. ( f ) Schematic of the experiment: a SCN2A-EGFP + HEK-293 cell was recorded in voltage-clamp and filled with OGB-1 (0.1 mM) after which a train of depolarizing pules (–120 to –30 mV, 200 Hz, 1 s) was applied, top to bottom , ΔF/F before and after bath application of 1 μM TTX (blue), the recorded currents and voltage command potentials, right , magnification of the first three action currents, scale bars from top to bottom represent 1% ΔF/F, 0.5 nA, 100 mV, 500 ms and 10 ms. ( g ) Color-coded average ΔF/F of 100 frames before onset ( left ) and at the end ( right ) of the voltage command, scale bar indicates 10 μm. ( h ) OGB-1 ΔF/F is significantly higher in control ( n = 7) than after application of TTX ( n = 4), one-tailed Mann Whitney test, p=0.0121. Data available in . Figure 7—source data 1. Na V 1.2 channels mediate a Ca 2+ influx.

Article Snippet: Gene (human) , SCN2A , Genscript, USA ( ) , , .

Techniques: Fluorescence, Transfection, Activation Assay, Control, One-tailed Test, MANN-WHITNEY

Journal: eLife

Article Title: Ca 2+ entry through Na V channels generates submillisecond axonal Ca 2+ signaling

doi: 10.7554/eLife.54566

Figure Lengend Snippet:

Article Snippet: Gene (human) , SCN2A , Genscript, USA ( ) , , .

Techniques: Plasmid Preparation, Software, Imaging